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Goat anti-Human Ig (Chain lambda), (Light Chain) Antibody (TRITC)

FACS, IF, IEP, IM Polyclonal TRITC F(ab')2 fragment
Catalog No. ABIN117724

Quick Overview for Goat anti-Human Ig (Chain lambda), (Light Chain) Antibody (TRITC) (ABIN117724)

Target

Ig

Fragment

F(ab')2 fragment

Clonality

  • 116
  • 95
Polyclonal

Conjugate

  • 53
  • 25
  • 23
  • 22
  • 18
  • 14
  • 12
  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
TRITC

Application

  • 166
  • 132
  • 95
  • 68
  • 57
  • 26
  • 22
  • 12
  • 12
  • 7
  • 6
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoelectrophoresis (IEP), Immunomicroscopy (IM)
  • Binding Specificity

    • 79
    • 78
    • 62
    • 34
    • 4
    • 2
    Chain lambda, Light Chain

    Reactivity

    • 114
    • 62
    • 19
    • 17
    • 9
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 96
    • 77
    • 38
    • 18
    • 3
    • 1
    • 1
    Goat

    Characteristics

    Absorption / Emission: 550 nm / 570 nm
    Molar Ratio: 4.0 moles TRITC per mole of Goat IgG F(ab')2).

    Purification

    Immunoaffinity chromatography.

    Immunogen

    Human lambda (lambda chain).
  • Application Notes

    Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence ofF(c) mediated binding, lot-to-lot consistency, high titer and specificity.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore with 0.4 mL of deionized water (or equivalent).

    Concentration

    2.5 mg/mL (by UV absorbance at 280 nm)

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Dilute only prior to immediate use

    Storage

    4 °C

    Storage Comment

    Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid. . DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  • Target

    Ig

    Alternative Name

    Immunoglobulin
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